Chromatography is a widely used laboratory technique for the separation of components in a mixture based on their differential interactions with the stationary phase. This technique aids in the identification and purification of components and is applicable for both qualitative and quantitative analysis.
Affinity chromatography is a type of liquid chromatography, which utilizes the reversible biological interaction (affinity) between components in the mobile phase and a solid stationary phase for separation.
- It was discovered by Pedro Cuatrecasas and Meir Wilcheck in 1968.
- Affinity chromatography falls under the…
