PCR involves three main steps: Denaturation, Annealing, and Extension. To make the method more efficient and specific, variations of the method have been developed, one of which is allele-specific PCR, used to amplify single-nucleotide polymorphisms.
Allele-Specific PCR (ASPCR) is a PCR method used to study mutations and polymorphisms by targeting specific alleles. This non-radioactive method can distinguish between alleles differing by one or more nucleotides based on whether amplification occurs during PCR.
In ASPCR, two sets of primers are used: one primer is specific to the mutant allele and will not…
