Maintaining bacterial stocks via frozen methods is a common way to preserve viable cultures. Frozen stocks may appear to be cost effective and user friendly, but freezing and thawing can create several problems. Some of these issues include:
- Time Consumption
- Cost Ineffectiveness
- Contamination
- Reduced Recovery
- Loss of Genetic Material & Expression
Despite these drawbacks, many laboratories continue to use frozen stocks, which may void product warranties and may compromise quality. Let’s discuss some of these concerns in further detail.
Time Consumption
It’s a common misconception that using frozen stock…
