Growth at 42°C Test is a physiological laboratory test used to identify and differentiate bacteria based on their ability to grow at 42°C temperature. It is done because 42°C is higher than the normal incubation temperature (35–37°C) and many bacteria cannot tolerate this heat. Bacteria that can grow at this temperature are considered to have heat tolerance factors like heat shock proteins which helps in survival and multiplication at thermal stress.

Objectives of Growth at 42°C Test
- To differentiate Pseudomonas species, mainly to separate Pseudomonas aeruginosa from…