Endospore staining is the special staining method used to differentiate the bacterial vegetative cell and its endospore. It is the process applied because some bacteria under harsh environmental conditions form a resistant structure known as the endospore.
It is a non-vegetative body and it is produced mainly by members of Bacillus spp and Clostridium spp when there is lack of nutrients, water, oxygen or when the temperature becomes unsuitable. These endospores have a tough outer covering which is made in such a way that it can resist high temperature, radiation and many chemicals, so the normal basic stains cannot enter inside.
It is the…
