Indirect ELISA (Enzyme-Linked Immunosorbent Assay) is a type of ELISA that is widely used for detecting and quantifying antibodies in samples such as serum and other biological fluids.
It is a two-step process, where a secondary antibody linked with an enzyme is used to detect the primary antibody produced against a specific antigen in the human body.
Principle of Indirect ELISA
Indirect ELISA relies on two antibodies: primary and secondary antibodies. In this assay, a specific antigen is coated on a 96-well plate, and the primary antibody produced in the human body against that antigen binds to it. This binding is…
